To investigate the relationship between the expression of RIOK1 and clinicopathological characteristics in NSCLC patients, we found that the expression of RIOK1 was correlated with pathological grade, tumor size, lymph node metastasis, and survival status of NSCLC (*p< 0.05, Table 1). This evidence concerns the gene RIOK1 and metastatic malignant neoplasm in the lymph nodes.