First, the “writer” genes NSUN1-NSUN7, the “eraser” genes TET2 and ALKBH1, and the “reader” genes ALYREF and YBX1 were significantly upregulated or downregulated in tumor tissues, suggesting these genes may be critical in m5C-related occurrence and progression of COAD. Here, ALYREF is linked to colon adenocarcinoma.