To evaluate the prognostic value of these 13 m5C-related regulators in COAD, univariate Cox regression analysis was used to identify m5C-related regulators that were highly correlated with the OS in patients with COAD, and two regulators with prognostic significance (p < 0.05) were found: NSUN6 and ALYREF. This evidence concerns the gene ALYREF and colon adenocarcinoma.