The data presented in this study, on the one hand, suggested the aberrant activated SP/NK1R signaling axis as a probable mediator of induction of oxidative stress in glioblastoma cells and, on the other hand, showed that aprepitant could exert anticancer effect on U87 cells by shifting the balance between oxidant and antioxidant components of the redox system. This evidence concerns the gene TFF2 and glioblastoma.