Further genetic assays unveiled that only the variant chr4:146,436,029C > A (GRCh37: NC_000004.11), equivalent to chr4:145,514,877C > A (GRCh38: NC_000004.12) or NM_005900.3: c.264C > A; p.(Tyr88∗), in SMAD1, was verified by Sanger sequencing with the primer pairs given in Table 3 and was shown to be in cosegregation with CHD in the entire family. The gene discussed is SMAD1; the disease is coronary artery disorder.