Interestingly, although artificially increasing intracellular [Ca2 +] does not change BBB TJ (Brown et al., 2004), the treatment with Ca2 + channel blocker SKF 96365 can improve the permeability increase effect of hypoxia/hyperglycemia on BBB through a mechanism that seems to involve the recruitment of occludin into the cell membrane (Brown and Davis, 2005). Here, OCLN is linked to Hyperglycemia.