Analysis of ferroptosis-related genes between the two groups revealed that many ferroptosis-related genes were differentially expressed between the two clusters (Figures 3C,E), and the expression levels of some genes (including ACSL5, ACSL1, GSS, SLC7A11, SCL39A8, SLC39A14, and PRNP) were significantly associated with the prognosis of patients with BLCA (Supplementary Figure S3). The gene discussed is ACSL1; the disease is bladder transitional cell carcinoma.