IHC assays were performed to evaluate COL8A1 expression levels in 94 NSCLC and 86 adjacent normal tissue samples and demonstrated that COL8A1 was aberrantly upregulated in NSCLC tissues, highlighting a potential role for COL8A1 as a novel prognostic biomarker of NSCLC, in which positive expression COL8A1 was associated with advanced pathological stage, consistent with a previous study (26). This evidence concerns the gene COL8A1 and non-small cell lung carcinoma.