established a radiation resistant H460 (RR-H460) cell line that displayed cancer stem like cell phenotypes from parental radiosensitive H460 non-small cell lung cancer cells, proteomic analyses of the two cell lines by LC-MS/MS revealed VIM, GRP78, fatty acid synthase (FASN), ubiquinol-cytochrome C reductase complex core protein 1 (UQCRC1), plasminogen activator inhibitor 2 (PAI-2), nodal modulator 2 (NOMO2), kinesin light chain 4 (KLC4) and procollagen-lysine 2-oxoglutarate 5-dioxygenase 3 (PLOD3) as radiation resistance biomarkers. The gene discussed is HSPA5; the disease is cancer.