These findings were further confirmed by western blot analysis, which investigated LMO1 expression in GBM cell lines (T98,LN229, U87 and U251 cell) and primary glioma cell lines (NFH-GBM1, NFH-GBM2, NFH-GBM3 cell) and normal glial cells (SVG cell) (Figure 1E). Here, NEFH is linked to central nervous system cancer.