TNFAIP6 and colorectal carcinoma: First, we established TSG-6 overexpressed CRC cell lines via transient plasmid transfection (HCT116 and DLD1) and observed the TSG-6 mRNA level in the cell lysate and supernatant protein level were dramatically increased in TSG-6 overexpressed cells compared with control cells (Figure S2B-S2D).