Using PKH-67 to label EVs, we observed that in the 2h group, in both EVs derived from alveolar macrophages and PyrBDs-rich samples from ALI mice, MLE-12 cells showed phagocytosis; the 4h group clearly showed micron-sized vesicle signal body in the cytoplasm. The gene discussed is PDXK; the disease is acute respiratory distress syndrome.