Immunofluorescent staining showed a strong cytoplasmic immunofluorescence signal for IL-6 (Figure 6A), IFN-γ (Figure 6B) and TNF-α (Figure 6E) and a minimal cytoplasmic immunofluorescence signal for IL-10 (Figure 6F) along with the marker of nuclei, DAPI (blue) (Figures 6C,G) in the epithelial cells of the ES from the sac specimens of patients with MD. This evidence concerns the gene TNF and Menkes disease.