No statistically significant differences in the expression levels of IFN-γ (P = 0.445), IL-10 (P = 0.332), IL-12p70 (P = 0.160), IL-17A (P = 0.464), IL-6 (P = 0.684) or TNF-α (P = 0.304) were identified in the serum comparing between the MD and AN groups; neither were there any statistical significant differences found in the expression levels of IFN-γ (P = 0.164), IL-10 (P = 0.444), IL-12p70 (P = 0.749), IL-17A (P = 0.323), IL-6 (P = 0.362) or TNF-α (P = 0.365) in the serum comparing between the MD group and the healthy control group (Figure 3B). This evidence concerns the gene TNF and Menkes disease.