NOS3 and diabetes mellitus: In contrast, VEGFKD significantly decreased GSNOR S-nitrosylation in eNOS−/−:VEGFKD (+dox) and in DM-VEGFKD (+dox) kidneys, as detected by biotin-shift assay (BST) (Figure 7B), This SNO-GSNOR reduction could decrease GSNOR activity and lead to GSNO accumulation, providing an alternate NO source (and might mitigate glomerular damage).