Detected number of CRH mRNA grains per cell (Figure 2C) showed significant reduction in GABAergic interneurons (control: 78.0 ± 3.5, MDD: 73.5 ± 3.9, p = 0.02; Figure 2D), but not in glutamatergic neurons (control: 86.0 ± 5.5, MDD: 95.4 ± 19.7, p = 0.67). This evidence concerns the gene CRH and major depressive disorder.