We explored the possible molecular mechanism of S100A16 in AKI via HRD1-mediated Wnt/β-catenin pathway activation by co-transfecting Ad-HRD1 and S100A16 overexpressing (OE) plasmids into NRK-49F cells to detect the protein expressions of GSK3β, CK1α, and HGF. This evidence concerns the gene GSK3B and acute kidney injury.