To determine whether MIR100HG regulates the EMT process in CRC cells, we specifically deleted the longest exon, exon 4, of MIR100HG (2638 bp) using CRISPR/Cas9 gene-editing (Supplementary Fig. 1c) in CC-CR cells; the resulting line is designated MIR100HGKOE4. Here, MIR100HG is linked to colorectal carcinoma.