To verify the correlation between SIRT5 expression level and PCa progression, an enzyme-linked immunosorbent assay (ELISA) was carried out to assess the levels of SIRT5 and Ksu in PCa tissues [benign prostatic hyperplasia (BPH) as control, T2 and T3 stages; Figure 1E]. This evidence concerns the gene SIRT5 and posterior cortical atrophy.