Though the serum BUN level was significantly higher in mice after subtotal nephrectomy, there was no statistically significant difference of BUN levels between ECJMJD3 KO mice and ECJMJD3+/+ mice observed either at 6 weeks or 10 weeks after CKD surgery, indicating that endothelial KO of JMJD3 did not aggravate renal dysfunction in mice (Fig. 3E). The gene discussed is KDM6B; the disease is chronic kidney disease.