Tumour cells from mice injected with siHNRNPC‐silenced PanC‐1/NF co‐cultures had significantly decreased HNRNPC, RhoA, ROCK2 and YAP expression, suggesting that radiation resistance is mediated via HNRNPC and the downstream RhoA/ROCK2/YAP signalling pathway in PC. This evidence concerns the gene HNRNPC and pachyonychia congenita.