To test our hypothesis, we first evaluated the dependence of PKCδ and NLRC4 phosphorylation and NLRC4 inflammasome activation on Crk in response to P. aeruginosa, by depleting BMDMs of Crk adaptor protein by Crk-specific siRNA9,82 prior to infection with P. aeruginosa. This evidence concerns the gene NLRC4 and infection.