Wounds were infected with 103 wild-type or T3SS mutant strains and the impact of infection with wild-type and T3SS mutants on the production of IL-1β and IL-18 pro-inflammatory chemokines (products of inflammasomes and important pro-inflammatory cytokines against P. aeruginosa infection55,56) was assessed by enzyme-linked immunosorbent assay (ELISA) 24 h after infection. This evidence concerns the gene IL18 and infection.