Moreover, infection with ExoT-deleted mutant strains resulted in nearly 3-fold increase in the number of NLRC4 inflammasome foci and complementing this strain with pExoT, but not empty vector, significantly reduced NLRC4 inflammasome foci numbers in BMDMs (Fig. 2i–j), indicating that ExoT interferes with NLRC4 inflammasome assembly. This evidence concerns the gene NLRC4 and infection.