In line with ExoT’s anti-inflammatory function in wound, infection with ExoT-deleted P. aeruginosa strains also resulted in substantial increases in caspase-1 activation as assessed by Western immunoblotting probing for the processed active caspase-1 fragment (p20) in BMDMs, and caspase-1 inflammasome assembly as determined by caspase-1/ASC foci number determination by immunofluorescent (IF) microscopy65 (Fig. 2e–f and Supplementary Fig. 3c–d). Here, CASP1 is linked to infection.