For example, expression difference of ATP11B, EIF4E, SQLE, MYO1B, RAP2B, TTC9, HECTD1, STRN, SDR9C7, RAB7A, LIMK2, FRMD6, and CLPX can be detected in hyperkeratosis with parakeratosis (Fig. 3A, Additional file 4: Figure S4A). The gene discussed is SQLE; the disease is Hyperkeratosis.