﻿Interestingly, TWIST1, a transcriptional target of MYCN, was found to be required for MYCN-dependent proliferation, hence, co-occupancy can be considered a deregulated MYCN-specific NB dependency.﻿ Conversely, loss of MYCN led to a global reduction in transcription, more evident at the MYCN target genes with the highest enhancer occupancy [23]. This evidence concerns the gene TWIST1 and neuroblastoma.