The KEGG pathway analysis was performed for 603 DEGs (758 DEGs were overlapped): 37 genes were classified into NAFLD pathway-genes involved in oxidative phosphorylation related reactions, including cytochrome c oxidase III (mt-Co3), nicotinamide adenine dinucleotide (NADH) dehydrogenase 3 (mt-Nd3), cytochrome c oxidase II (mt-Co2), NADH dehydrogenase 4l (mt-Nd4l), cytochrome b (mt-Cytb), and adenosine triphosphate synthase 6 (mt-Atp6), which were downregulated in mice fed with mixed oil. The gene discussed is MT-ND3; the disease is metabolic dysfunction-associated steatotic liver disease.