The lard and soybean oil mixture alleviated NAFLD by downregulating Fabp2, Fas, Traf2, Ap-1, mt-Cytb, Il-6, and Il-1 genes, upregulating Ampkα2 and Hsl genes, and promoting conjugated BAs and BAs signal receptor TGR5 protein. This evidence concerns the gene PRKAA2 and metabolic dysfunction-associated steatotic liver disease.