When benzyl isothiocyanate was administrated to Kupffer cell cultures (2.5 or 5.0 μM benzyl isothiocyanate) or male C57BL/6 J mice with induced NASH (1 g/kg/d benzyl isothiocyanate for 9 weeks) (80 mg/kg HED), a reduction in NLRP3 inflammasome activation was observed, through a reduction in NLRP3, p20 caspase-1, and IL1-β expression in blood and liver; moreover, benzyl isothiocyanate has shown to reduce the release of cathepsin β, an inflammasome assembler, and the interaction of cathepsin B with NLRP3. The gene discussed is CTSB; the disease is metabolic dysfunction-associated steatohepatitis.