Through gene expression and Western-blot analysis the authors were able to conclude that all diets (olive oil, omega-3 and omega-6) exhibited anti-inflammatory effects in the adipose tissue by inducing CB2 and upregulating nitric oxide synthase 1 (NOS1), both participating in the prevention of diet-induced obesity. This evidence concerns the gene CNR2 and obesity due to melanocortin 4 receptor deficiency.