In addition to the bioinformatics data (Electronic Supplementary Material Fig.1), the RT-PCR results demonstrated the amplification of the NANOG fragment from NTERA2 cDNA (Figure 1), which expresses NANOG but not NANOGP8.16 Early pluripotency signatures were reported by Kashyap et al. for genes regulated by OCT4, SOX2, and NANOG.52 In the present work, two of the three master regulators of pluripotency expressed in human glioblastoma were found, as well as increased expression of CARM1, which is involved in the regulation of both factors.44 This evidence concerns the gene POU5F1 and glioblastoma.