Figure 2D shows representative tumors with lectin-painted blood vessels co-stained for the blood vessel marker CD31. The indicated micrographs show a lack of lectin-staining in HCC with intact ECM, even though the tumors were positive for vessel staining (CD31). TNFα-CSG-treated tumors show visible lectin-painted blood vessels (lectin+/CD31+). The quantitative analysis showed a 6-fold increase in lectin-painted tumor blood vessels in TNFα-CSG-treated HCC (Figure 2D). This evidence concerns the gene TNF and hepatocellular carcinoma.