Seventeen patients (17%) were tested more than seven days before the study inclusion because of the following reasons: rescheduling of cancer treatment, without repeating RT-PCR by decision of the attending physician (n=4); urgent change of therapy on the day of inclusion without the possibility of repeating the test (n=1); patient refusal to repeat the test (n=2); patient under single agent anti-PD-1/PD-L1 immunotherapy (n=2); and reason not documented (n=8). The gene discussed is CD274; the disease is cancer.