To further investigate the potential genetic pathological processes of AF and provide new noninvasive methods for the clinical diagnosis and treatment of this disease, the downregulated (CHRNA5, LOC150051, and PP12719) and upregulated (DHRS9, LOC101928304, and RYR1) hub genes were demonstrated to be involved in the regulation of multiple pathways and to have strong predictive power for the occurrence of AF. This evidence concerns the gene CHRNA5 and atrial fibrillation.