Similar to other studies using different cell lines (Bandiera et al., 2011a,b; Das et al., 2012; Sripada et al., 2012; Tattikota et al., 2013; Zhang et al., 2014; Wang et al., 2015; Bose et al., 2020), we found that both components of the miRNA pathway, Dicer and Ago2, can be located in the mitochondria of osteosarcoma 143B cybrids. Here, AGO2 is linked to osteosarcoma.