In HCC TIL-CD8+ T cells, TPX2 overexpression dramatically increased the phosphorylation of P65; in human PBMC CD8+ T cells, TPX2 downregulation significantly reduced the phosphorylation of P65, but no change in NF-κB expression was observed (Fig. 6C). This evidence concerns the gene NFKB1 and hepatocellular carcinoma.