It has been reported that Notch1 expression is directly associated with the metastasis and decreased survival of a mouse MB model, and as a pivotal driver of metastasis and self-renewal of human Group 3 MB cells.72 Meanwhile, other research also showed that Notch2 but not Notch1, 3, 4 was overexpressed in MBs.73 Our study demonstrates that Notch2 was the main molecule involved in the pathway of Kir2.1 in non-WNT/SHH MBs, emphasizing its critical involvement in the progression of MBs. This evidence concerns the gene NOTCH1 and Mobius syndrome.