NANOG and amyotrophic lateral sclerosis: These ALS-iPSC lines were confirmed for their markers of pluripotency and then differentiated into MNPs and MNs, in parallel with normal, wild-type SOD1 human iPSCs (WTC11), as confirmed by the immuno-detection of OLIG2, Tuj1, and HB9 (Additional file 2: Figure S4a) and by qPCR on OCT4, NANOG, OLIG2, HB9, and CHAT (Additional file 2: Figure S4b).