Despite not finding an overall difference in HSC‐associated genes between CD34‐positive and CD34‐negative samples, we did find enrichment in CD34‐positive leukemias of HSC‐associated genes in a rank‐based GSEA as well as upregulation of key individual genes, previously described to be associated with HSC in several studies, such as the gene encoding the growth guidance receptor ROBO4, which is expressed on HSC as well as AML LSC [63], and plays a role in healthy HSC trafficking [64, 65, 66, 67]. Here, ROBO4 is linked to acute myeloid leukemia.