Mood States: POMS (anxiety (+), depression (+), anger (+), confusion (/), vigor (+), fatigue (+));Inflammatory cytokine: IL-6 (+), TNF-α (+);Oxidative stress: T-SOD (/), MDA (+);Cardiovascular disease risk biomarkers: ET-1 (+), Platelet activation (/);Immunocytes: Total T cell (/), Total B cell (+), Thlymphocyte (/), Tslymphocyte (/), NK cell (/), CD4/CD8(/);Serum cortisol (+), testosterone (/). The gene discussed is EDN1; the disease is major depressive disorder.