Another study conducted in mice with DSS-induced colitis showed decreased levels of pro-inflammatory cytokines (IL-1β, TNF, and IL-13 and NF-κB) and increased expression of TJ proteins (claudin-1, claudin-2, occludin and ZO-1) after administration of C. butryricum in the amount of 108 CFU for 7 days [63]. This evidence concerns the gene TJP1 and colitis.