In contrast to the favorable effect of estradiol on aortic strain and expression of Nos3, there was no effect of the ovariectomy and/or estradiol substitution on the expression of also “cardiovascular” gene for Cx37 [18,19], which is potential target for intervention in cardiovascular disease [20] and described as important for cardiovascular disease in previous (but not all) studies [21,22,23,24]. This evidence concerns the gene NOS3 and cardiovascular disorder.