INS and type 2 diabetes mellitus: By the use of the hyperinsulinemic-euglycemic clamp technique, Haus et al. showed that insulin sensitivity resulted as lower in type 2 diabetes subjects, and that the concentrations of C18:0, C20:0, C24:1 and total ceramides were significantly higher compared to controls, while insulin sensitivity was inversely correlated with C18:0, C20:0, C24:1, C24:0, and total ceramides [69].