TNF and Miyoshi myopathy: Real-time qRT-PCR analyses were performed to examine the expression patterns of the cytokine (interleukin and tumor necrosis factor) and chemokine receptors of primary tumors (superficial spreading melanoma (SSM), n = 7; nodular melanoma (NM), n = 3) and metastasis-originated (MM, n = 9) melanoma cell lines.