INS and polycystic ovary syndrome: In examining the acute increase in circulating insulin levels following daily insulin injections (during the 3rd–5th 30 day period or menstrual cycle at approximately 3–5 months of treatment), circulating insulin levels rose (p ≤ 0.02, Bonferroni corrected for six comparisons) above morning basal levels by ~364% and ~32% in control and PCOS-like females, respectively (Table 2), 4 h after daily insulin injections.