Overall duration of menstrual cycles remained the same (Figure 4; control females: diluent, 28.8 ± 3.1 days; insulin; 27.4 ± 1.0 days; PCOS-like females: diluent, 34.7 ± 2.6 days, insulin: 28.7 ± 1.0 days). This evidence concerns the gene INS and polycystic ovary syndrome.