We induced Ang II-induced hypertension in male and female wild-type, global Agtr1a-/-, and proximal tubule-specific Agtr1a-/- mice by infusing exogenous Ang II (0.5–1.5 mg/kg/day, i.p.)or by adenovirus-mediated overexpressing an intracellular cyan fluorescent protein tagged Ang II fusion protein, Ad-sglt2-ECFP/Ang II, selectively in the proximal tubules using the SGLT2 promoter [16,28]. The gene discussed is AGT; the disease is Hypertension.