Exposure of PHH, HepaRG, and hSKP-HPC to ‘NASH’ triggers results in predicted activation of NASH-specific canonical pathways, including HMGB1 and triggering receptor expressed on myeloid cells-1 (TREM1)-signaling that are predicted as being activated in all three in vitro NASH datasets and at least one NASH patient dataset (Figure 3B). The gene discussed is HMGB1; the disease is metabolic dysfunction-associated steatohepatitis.