To establish an intelligent and automated method for accurate differentiation of HDs, E/NSCLC, and A/NSCLC, EGFR and CXCR4 expression in sEVs were used as machine learning objects, and sera sEVs from 51 individuals were randomly divided into the training cohort (39 samples) and validation cohort (12 samples). Here, EGFR is linked to non-small cell lung carcinoma.