DHFR and cancer: In addition, a molecular docking study was carried out to understand the potential mechanism of action on five molecular targets involved in survival and proliferation of cancer cells: topoisomerase IIα (TIIα), topoisomerase IIβ (TIIβ) dihydrofolate reductase (DHFR), methylenetetrahydrofolate dehydrogenase (MTHFD), and Bcl-2-related protein A1 (BCL-2).