In this work, we analyzed, by an immunohistochemical approach, the lymph node biopsies derived from FL patients at grades 1,2, and 3A, at first diagnosis and focused our attention on the quantification of tumor microenvironmental cells, including macrophages, mast cells, CD4+ and CD8+ lymphocytes, and PD-L1 positive cells and the microvessel density in the different groups of patients comparing them with normal lymph nodes. The gene discussed is CD4; the disease is neoplasm.