The meta-analyses conducted to assess the effects of probiotic supplementation on GCF levels of IL-1β (p-value = 0.393 and n = three studies), IL-8 (p-value = 0.434 and n = two studies), IL-10 (p-value = 0.902 and n = two studies), and TNF-α (p-value = 0.495 and n = three studies) in periodontal disease patients were not statistically significant when compared to controls. The gene discussed is TNF; the disease is periodontal disorder.