While Nrf2 gene expression and protein levels were unaffected in HF + EtOH diet fed mice, it is important to note that Nrf2 activation is controlled by various factors, including Keap1-mediated degradation and post-transcriptional regulation by microRNAs, binding partners, alternative splicing, epigenetic modifications, phosphorylation, and subcellular localization [43]. This evidence concerns the gene KEAP1 and hydrops fetalis.