These results were further validated in a Japanese cohort of 92 JMML patients, in which the CpG methylation status of 16 genes was analyzed, including nine genes that were common to the European study (CALCA, CDKN2B, DAPK1, MGMT, MLH1, RARB, RASSF1, TP73, and BMP4) and seven new candidate genes (APC, CDH13, CDKN1A, CHFR, ESR1, H19, and IGF2AS). The gene discussed is CALCA; the disease is juvenile myelomonocytic leukemia.