Histology and NGS allowed the distinction of five tumor subgroups: glioblastoma IDH wildtype (GBM); diffuse midline glioma H3 K27M-mutated (DMG-H3); high-grade astrocytoma with piloid features (HAP); diffuse astrocytoma IDH mutated (DA), diffuse leptomeningeal glioneuronal tumors (DGLN) and pilocytic astrocytoma (PA). Here, IDH1 is linked to diffuse midline glioma.