Compared to PITX1 negative, low or high PITX1 level (in the following denoted as samples with a positive PITX1 level) was associated with higher tumor aggressiveness including advanced tumor stage (p-value < 0.0001), higher Gleason grade, more presence of lymph node metastasis, and more positive surgical margin (p-value < 0.0001 each). This evidence concerns the gene PITX1 and metastatic malignant neoplasm in the lymph nodes.